A Cell-Free Protein Synthesis Platform to Produce a Clinically Relevant Allergen Panel.
ACS Synth Biol
; 12(8): 2252-2261, 2023 08 18.
Article
in En
| MEDLINE
| ID: mdl-37553068
ABSTRACT
Allergens are used in the clinical diagnosis (e.g., skin tests) and treatment (e.g., immunotherapy) of allergic diseases. With growing interest in molecular allergy diagnostics and precision therapies, new tools are needed for producing allergen-based reagents. As a step to address this need, we demonstrate a cell-free protein synthesis approach for allergen production of a clinically relevant allergen panel composed of common allergens spanning a wide range of phylogenetic kingdoms. We show that allergens produced with this approach can be recognized by allergen-specific immunoglobulin E (IgE), either monoclonals or in patient sera. We also show that a cell-free expressed allergen can activate human cells such as peripheral blood basophils and CD34+ progenitor-derived mast cells in an IgE-dependent manner. We anticipate that this cell-free platform for allergen production will enable diagnostic and therapeutic technologies, providing useful tools and treatments for both the allergist and allergic patient.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Immunoglobulin E
/
Allergens
Limits:
Humans
Language:
En
Journal:
ACS Synth Biol
Year:
2023
Document type:
Article
Affiliation country: