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Engineered bacteria detect tumor DNA.
Cooper, Robert M; Wright, Josephine A; Ng, Jia Q; Goyne, Jarrad M; Suzuki, Nobumi; Lee, Young K; Ichinose, Mari; Radford, Georgette; Ryan, Feargal J; Kumar, Shalni; Thomas, Elaine M; Vrbanac, Laura; Knight, Rob; Woods, Susan L; Worthley, Daniel L; Hasty, Jeff.
Affiliation
  • Cooper RM; Synthetic Biology Institute, University of California, San Diego, La Jolla, CA 92093, USA.
  • Wright JA; Precision Cancer Medicine Theme, South Australia Health and Medical Research Institute, Adelaide, SA 5000, Australia.
  • Ng JQ; Adelaide Medical School, University of Adelaide, Adelaide, SA 5000, Australia.
  • Goyne JM; Precision Cancer Medicine Theme, South Australia Health and Medical Research Institute, Adelaide, SA 5000, Australia.
  • Suzuki N; Precision Cancer Medicine Theme, South Australia Health and Medical Research Institute, Adelaide, SA 5000, Australia.
  • Lee YK; Adelaide Medical School, University of Adelaide, Adelaide, SA 5000, Australia.
  • Ichinose M; Adelaide Medical School, University of Adelaide, Adelaide, SA 5000, Australia.
  • Radford G; Precision Cancer Medicine Theme, South Australia Health and Medical Research Institute, Adelaide, SA 5000, Australia.
  • Ryan FJ; Adelaide Medical School, University of Adelaide, Adelaide, SA 5000, Australia.
  • Kumar S; Adelaide Medical School, University of Adelaide, Adelaide, SA 5000, Australia.
  • Thomas EM; Precision Cancer Medicine Theme, South Australia Health and Medical Research Institute, Adelaide, SA 5000, Australia.
  • Vrbanac L; Flinders Health and Medical Research Institute, Flinders University, Bedford Park, SA 5042, Australia.
  • Knight R; Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA.
  • Woods SL; Adelaide Medical School, University of Adelaide, Adelaide, SA 5000, Australia.
  • Worthley DL; Adelaide Medical School, University of Adelaide, Adelaide, SA 5000, Australia.
  • Hasty J; Molecular Biology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA.
Science ; 381(6658): 682-686, 2023 08 11.
Article in En | MEDLINE | ID: mdl-37561843
ABSTRACT
Synthetic biology has developed sophisticated cellular biosensors to detect and respond to human disease. However, biosensors have not yet been engineered to detect specific extracellular DNA sequences and mutations. Here, we engineered naturally competent Acinetobacter baylyi to detect donor DNA from the genomes of colorectal cancer (CRC) cells, organoids, and tumors. We characterized the functionality of the biosensors in vitro with coculture assays and then validated them in vivo with sensor bacteria delivered to mice harboring colorectal tumors. We observed horizontal gene transfer from the tumor to the sensor bacteria in our mouse model of CRC. This cellular assay for targeted, CRISPR-discriminated horizontal gene transfer (CATCH) enables the biodetection of specific cell-free DNA.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Acinetobacter / DNA, Neoplasm / Biosensing Techniques / Colorectal Neoplasms / Cell-Free Nucleic Acids Type of study: Diagnostic_studies / Prognostic_studies Limits: Animals / Humans Language: En Journal: Science Year: 2023 Document type: Article Affiliation country:
Fulltext
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PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Acinetobacter / DNA, Neoplasm / Biosensing Techniques / Colorectal Neoplasms / Cell-Free Nucleic Acids Type of study: Diagnostic_studies / Prognostic_studies Limits: Animals / Humans Language: En Journal: Science Year: 2023 Document type: Article Affiliation country: