Variations of Redox Balance in Different Stages of Childhood Immune Thrombocytopenic Purpura.
Thromb Haemost
; 123(12): 1129-1139, 2023 Dec.
Article
in En
| MEDLINE
| ID: mdl-37604187
ABSTRACT
BACKGROUND:
Few previous studies indicated the role of oxidative stress in the pathogenesis of childhood idiopathic thrombocytopenic purpura (ITP), but there are little data regarding changes in redox balance in different forms of the disease, and changes after therapeutic procedures. We aimed to investigate the values of pro-oxidants and antioxidative capacity in various forms of ITP before and after the applying therapy. MATERIALS ANDMETHODS:
The research included 102 children, classified into the following groups (1) newly diagnosed ITP (ndITP), (2) persistent ITP, (3) chronic ITP (chITP), and (4) control groups (A) healthy control and (B) previously experienced ITP-healthy children who had been suffering from ITP earlier. During the clinical assessment, a blood sample was taken from the patients, from which the value of pro-oxidants (index of lipid peroxidation measured as TBARS, nitrites [NO2 -], as measurement of nitric oxide [NO] production, superoxide anion radical [O2 -], and hydrogen peroxide [H2O2]) and the capacity of antioxidant protection (activity of superoxide dismutase and catalase, and quantity of reduced glutathione) were determined spectrophotometrically.RESULTS:
Our results demonstrated that values of pro-oxidants, especially reflected through the TBARS and O2 -, were the highest in the ndITP and exacerbated chITP groups. Also, the activity of the endogenous antioxidative defense system was the lowest in these groups. Intravenous immunoglobulin therapy in the ndITP group exerted the most prominent effect on the redox balance.CONCLUSION:
It can be concluded that severity and exacerbation of the ITP are closely related to the redox status.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Purpura, Thrombocytopenic, Idiopathic
Limits:
Child
/
Humans
Language:
En
Journal:
Thromb Haemost
Year:
2023
Document type:
Article