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Establishment of transgenic epithelium-specific Cre-recombinase driving medaka (Oryzias latipes) by homology repair mediated knock-in.
Watanabe, Yuko; Katsumura, Eri; Domon, Tatsuki; Ishikawa, Yuta; Oguri, Rina; Takashima, Minami; Meng, Qi; Kinoshita, Masato; Hashimoto, Hisashi; Hitomi, Kiyotaka.
Affiliation
  • Watanabe Y; Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya, Japan.
  • Katsumura E; Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya, Japan.
  • Domon T; Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya, Japan.
  • Ishikawa Y; Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya, Japan.
  • Oguri R; Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya, Japan.
  • Takashima M; Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya, Japan.
  • Meng Q; Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya, Japan.
  • Kinoshita M; Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
  • Hashimoto H; Graduate School of Science, Nagoya University, Nagoya, Japan.
  • Hitomi K; Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya, Japan.
Biosci Biotechnol Biochem ; 87(11): 1285-1294, 2023 Oct 25.
Article in En | MEDLINE | ID: mdl-37607777
Deletion of gene expression in the target tissues and cells is an effective strategy for elucidating the physiological functions of the protein of interest. For tissue-specific and/or inducible gene deletion, the Cre-loxP system has been widely used in various model organisms including medaka (Oryzias latipes). The epithelium is the key tissue, locating at the outermost area and playing a role in barrier to external stimuli. Despite a large genetic toolbox developed in medaka, there is no available Cre-driver line that works in an epithelium-specific manner. Here, we established epithelium-specific Cre-driver lines in medaka using a homology-directed repair mediated knock-in approach with CRISPR/Cas9, targeting each of periplakin and keratin genes. We show that Cre-recombinase is expressed exclusively in the epithelium in the knock-in lines and that it efficiently and specifically induces recombination in the tissues. These Cre-driver lines are useful for studying the functions of proteins expressed in the epithelium.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oryzias Limits: Animals Language: En Journal: Biosci Biotechnol Biochem Journal subject: BIOQUIMICA / BIOTECNOLOGIA Year: 2023 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oryzias Limits: Animals Language: En Journal: Biosci Biotechnol Biochem Journal subject: BIOQUIMICA / BIOTECNOLOGIA Year: 2023 Document type: Article Affiliation country: Country of publication: