An increase in Wharton's jelly membrane osteocompatibility by a genipin-cross-link.
Int J Biol Macromol
; 255: 127562, 2024 Jan.
Article
in En
| MEDLINE
| ID: mdl-37865356
ABSTRACT
Wharton's Jelly (WJ) has attracted significant interest in the field of tissue healing thanks to its biological properties, including antibacterial activity and immunomodulation. However, due to the fast degradation and poor mechanical behavior in biological environment, its application in bone regeneration is compromised. Here, we proposed to use genipin as an efficient cross-linking agent to significantly improve the elasticity and the enzymatical stability of the WJ matrix. The degree of cross-linking, linear elastic moduli, and collagenase resistance varied over a wide range depending on genipin concentration. Furthermore, our results highlighted that an increase in genipin concentration led to a decreased surface wettability, therefore impairing cell attachment and proliferation. The genipin cross-linking prevented rapid in vitro and in vivo degradation, but led to an adverse host reaction and calcification. When implanted in the parietal bone defect, a limited parietal bone regeneration to the dura was observed. We conclude that genipin-cross-linked WJ is a versatile medical device however, a careful selection is required with regards to the genipin concentration.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Wharton Jelly
/
Mesenchymal Stem Cells
Language:
En
Journal:
Int J Biol Macromol
Year:
2024
Document type:
Article
Affiliation country: