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Mapping stress inside living cells by atomic force microscopy in response to environmental stimuli.
Wang, Hongxin; Zhang, Han; Tamura, Ryo; Da, Bo; Abdellatef, Shimaa A; Watanabe, Ikumu; Ishida, Nobuyuki; Fujita, Daisuke; Hanagata, Nobutaka; Nakagawa, Tomoki; Nakanishi, Jun.
Affiliation
  • Wang H; Research Center for Macromolecules and Biomaterials, National Institute for Materials Science, Tsukuba, Ibaraki, Japan.
  • Zhang H; Center for Basic Research on Materials, National Institute for Materials Science, Tsukuba, Ibaraki, Japan.
  • Tamura R; Center for Basic Research on Materials, National Institute for Materials Science, Tsukuba, Ibaraki, Japan.
  • Da B; Center for Basic Research on Materials, National Institute for Materials Science, Tsukuba, Ibaraki, Japan.
  • Abdellatef SA; Research Center for Macromolecules and Biomaterials, National Institute for Materials Science, Tsukuba, Ibaraki, Japan.
  • Watanabe I; Center for Basic Research on Materials, National Institute for Materials Science, Tsukuba, Ibaraki, Japan.
  • Ishida N; Center for Basic Research on Materials, National Institute for Materials Science, Tsukuba, Ibaraki, Japan.
  • Fujita D; Center for Basic Research on Materials, National Institute for Materials Science, Tsukuba, Ibaraki, Japan.
  • Hanagata N; Research Network and Facility Services Division, National Institute for Materials Science, Tsukuba, Ibaraki, Japan.
  • Nakagawa T; Department of Diagnostic Pathology, University of Tsukuba Hospital, Tsukuba, Ibaraki, Japan.
  • Nakanishi J; Research Center for Macromolecules and Biomaterials, National Institute for Materials Science, Tsukuba, Ibaraki, Japan.
Sci Technol Adv Mater ; 24(1): 2265434, 2023.
Article in En | MEDLINE | ID: mdl-37867575
The response of cells to environmental stimuli, under either physiological or pathological conditions, plays a key role in determining cell fate toward either adaptive survival or controlled death. The efficiency of such a feedback mechanism is closely related to the most challenging human diseases, including cancer. Since cellular responses are implemented through physical forces exerted on intracellular components, more detailed knowledge of force distribution through modern imaging techniques is needed to ensure a mechanistic understanding of these forces. In this work, we mapped these intracellular forces at a whole-cell scale and with submicron resolution to correlate intracellular force distribution to the cytoskeletal structures. Furthermore, we visualized dynamic mechanical responses of the cells adapting to environmental modulations in situ. Such task was achieved by using an informatics-assisted atomic force microscope (AFM) indentation technique where a key step was Markov-chain Monte Carlo optimization to search for both the models used to fit indentation force-displacement curves and probe geometry descriptors. We demonstrated force dynamics within cytoskeleton, as well as nucleoskeleton in living cells which were subjected to mechanical state modulation: myosin motor inhibition, micro-compression stimulation and geometrical confinement manipulation. Our results highlight the alteration in the intracellular prestress to attenuate environmental stimuli; to involve in cellular survival against mechanical signal-initiated death during cancer growth and metastasis; and to initiate cell migration.
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Sci Technol Adv Mater Year: 2023 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Sci Technol Adv Mater Year: 2023 Document type: Article Affiliation country: Country of publication: