PMCA screening of retropharyngeal lymph nodes in white-tailed deer and comparisons with ELISA and IHC.
Sci Rep
; 13(1): 20171, 2023 11 17.
Article
in En
| MEDLINE
| ID: mdl-37978312
ABSTRACT
Chronic wasting disease (CWD) is a prion disease affecting cervids. CWD diagnosis is conducted through enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry (IHC) in retropharyngeal lymph nodes. Unfortunately, these techniques have limited sensitivity against the biomarker (CWD-prions). Two in vitro prion amplification techniques, real-time quaking-induced conversion (RT-QuIC) and protein misfolding cyclic amplification (PMCA), have shown promise in detecting CWD-prions in tissues and bodily fluids. Recent studies have demonstrated that RT-QuIC yields similar results compared to ELISA and IHC. Here, we analyzed 1003 retropharyngeal lymph nodes (RPLNs) from Texas white-tailed deer. PMCA detected CWD at a higher rate compared to ELISA/IHC, identified different prion strains, and revealed the presence of CWD-prions in places with no previous history. These findings suggest that PMCA exhibits greater sensitivity than current standard techniques and could be valuable for rapid and strain-specific CWD detection.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Prions
/
Deer
/
Wasting Disease, Chronic
Limits:
Animals
Language:
En
Journal:
Sci Rep
Year:
2023
Document type:
Article
Affiliation country: