The role of lysosomes as intermediates in betacoronavirus PHEV egress from nerve cells.
J Virol
; 97(12): e0133823, 2023 Dec 21.
Article
in En
| MEDLINE
| ID: mdl-38009916
ABSTRACT
IMPORTANCE Betacoronaviruses, including severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and mouse hepatitis virus (MHV), exploit the lysosomal exocytosis pathway for egress. However, whether all betacoronaviruses members use the same pathway to exit cells remains unknown. Here, we demonstrated that porcine hemagglutinating encephalomyelitis virus (PHEV) egress occurs by Arl8b-dependent lysosomal exocytosis, a cellular egress mechanism shared by SARS-CoV-2 and MHV. Notably, PHEV acidifies lysosomes and activates lysosomal degradative enzymes, while SARS-CoV-2 and MHV deacidify lysosomes and limit the activation of lysosomal degradative enzymes. In addition, PHEV release depends on V-ATPase-mediated lysosomal pH. Furthermore, this is the first study to evaluate ßCoV using lysosome for spreading through the body, and we have found that lysosome played a critical role in PHEV neural transmission and brain damage caused by virus infection in the central nervous system. Taken together, different betacoronaviruses could disrupt lysosomal function differently to exit cells.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Coronavirus Infections
/
Exocytosis
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Betacoronavirus 1
/
Lysosomes
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Neurons
Limits:
Animals
Language:
En
Journal:
J Virol
Year:
2023
Document type:
Article
Affiliation country: