Function analysis of fish PACT gene in response to virus infection.
Fish Shellfish Immunol
; 144: 109304, 2024 Jan.
Article
in En
| MEDLINE
| ID: mdl-38103849
ABSTRACT
PACT (interferon-inducible double-stranded RNA-dependent protein kinase activator A) is a cellular protein which can activate PKR in dsRNA-independent manner. However, the role of PACT in fish virus infection remains largely unknown. In this study, a PACT homologue from grouper (Epinephelus coioides)(EcPACT) was cloned and characterized. The open reading frame of EcPACT has a full length of 924 bp and encodes a protein of 307 amino acids with a predicted molecular weight of 33.29 kDa. Similar to mammals, EcPACT contains three dsRBD domains. EcPACT shares 99.67 % homology with E. lanceolatus. Real-time fluorescence quantitative PCR results showed that EcPACT mRNA was widely expressed in all tissues and abundantly expressed in brain, blood, head kidney and kidney. In addition, SGIV and RGNNV infection significantly upregulated the transcript levels of EcPACT. Subcellular localization analysis showed that EcPACT was mainly distributed in the nucleus. Overexpression of EcPACT inhibited the replication of SGIV and RGNNV in vitro and positively regulated the expression of interferon (IFN) and pro-inflammatory factors. The results provide a better understanding of the relationship between PACT and viral infection in fish.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bass
/
Virus Diseases
/
DNA Virus Infections
/
Fish Diseases
Limits:
Animals
Language:
En
Journal:
Fish & shellfish immunology (Online)
/
Fish Shellfish Immunol
/
Fish shellfish immunol
Journal subject:
BIOLOGIA
/
MEDICINA VETERINARIA
Year:
2024
Document type:
Article
Affiliation country:
Country of publication: