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An optimal skeletal element for DNA testing: Evaluation of DNA quantity and quality from various bone types in routine forensic practice.
Otagiri, Tomomi; Sato, Noriko; Shiozaki, Tetsuya; Harayama, Yuta; Matsumoto, Momoe; Kobayashi, Kanya; Asamura, Hideki.
Affiliation
  • Otagiri T; Department of Legal Medicine, Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan. Electronic address: 20hm108b@shinshu-u.ac.jp.
  • Sato N; Department of Legal Medicine, Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan. Electronic address: nosato@shinshu-u.ac.jp.
  • Shiozaki T; Department of Legal Medicine, Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan. Electronic address: tshiozaki@shinshu-u.ac.jp.
  • Harayama Y; Department of Legal Medicine, Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan. Electronic address: yharayama@shinshu-u.ac.jp.
  • Matsumoto M; Department of Legal Medicine, Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan. Electronic address: 22ms009b@shinshu-u.ac.jp.
  • Kobayashi K; Department of Legal Medicine, Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan. Electronic address: kanya_k@shinshu-u.ac.jp.
  • Asamura H; Department of Legal Medicine, Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan. Electronic address: asamura@shinshu-u.ac.jp.
Leg Med (Tokyo) ; 68: 102415, 2024 May.
Article in En | MEDLINE | ID: mdl-38280273
ABSTRACT
For human identification, the quality and quantity of DNA must be sufficient for amplification and analysis. When DNA extraction from bone tissues and teeth is required, the optimal skeletal elements should be selected as samples for DNA extraction because DNA yield differs among elements. Recently, some studies have reported that a high quantity of high-quality DNA can be extracted from the small cancellous bones of the hands and feet. In this study, we evaluated the effectiveness of small cancellous bones in the human identification of skeletal remains in routine forensic genetic casework. Cancellous bones [phalanges, (meta)carpal bones, and (meta)tarsal bones)] and the cortical bones (femur and petrous bones) and teeth, which have generally been recommended as samples, were collected from the same individuals that needed identifying using DNA analysis in our laboratory. The quantity of DNA from small cancellous bones tended to be higher than that from cortical bones, and the quality from the former was as high as that from the latter. This study showed that in routine forensic casework, the small cancellous bones of the hands and feet should be actively selected as samples for DNA testing.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA Type of study: Evaluation_studies Limits: Adult / Aged / Female / Humans / Male / Middle aged Language: En Journal: Leg Med (Tokyo) Journal subject: JURISPRUDENCIA Year: 2024 Document type: Article Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA Type of study: Evaluation_studies Limits: Adult / Aged / Female / Humans / Male / Middle aged Language: En Journal: Leg Med (Tokyo) Journal subject: JURISPRUDENCIA Year: 2024 Document type: Article Country of publication: