In vitro immune-enhancing effects of Platycodon grandiflorum combined with Salvia plebeian via MAPK and NF-κB signaling in RAW264.7 cells.
PLoS One
; 19(2): e0297512, 2024.
Article
in En
| MEDLINE
| ID: mdl-38306362
ABSTRACT
The immune-enhancing activity of the combination of Platycodon grandiflorum and Salvia plebeian extracts (PGSP) was evaluated through macrophage activation using RAW264.7 cells. PGSP (250-1000 µg/mL) showed a higher release of NO in a dose-dependent manner. The results showed that PGSP could significantly stimulate the production of PGE2, COX-2, TNF-α, IL-1ß, and IL-6 in RAW264.7 cells and promote iNOS, COX-2, TNF-α, IL-1ß, IL-4, and IL-6 mRNA expression. Western blot analysis demonstrated that the protein expression of iNOS and COX-2 and the phosphorylation of ERK, JNK, p38, and NF-κB p65 were greatly increased in PGSP-treated cells. PGSP also promoted the phagocytic activity of RAW264.7 cells. All these results indicated that PGSP might activate macrophages through MAPK and NF-κB signaling pathways. Taken together, PGSP may be considered to have immune-enhancing activity and might be used as a potential immune-enhancing agent.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Salvia
/
Platycodon
Limits:
Animals
Language:
En
Journal:
PLoS ONE (Online)
/
PLoS One
/
PLos ONE
Journal subject:
CIENCIA
/
MEDICINA
Year:
2024
Document type:
Article
Country of publication: