Protocol for culturing and functionally manipulating planarian neoblasts using SiR-DNA-based flow cytometry.
STAR Protoc
; 5(1): 102877, 2024 Mar 15.
Article
in En
| MEDLINE
| ID: mdl-38324448
ABSTRACT
Neoblasts are the only cells capable of proliferation in planarians. The traditional flow cytometry protocol using Hoechst inhibits the cell cycle. Here, we present a protocol for culturing and functionally manipulating planarian neoblasts using SiR-DNA-based flow cytometry. We describe steps for cell dissociation and staining, flow cytometry, and cell collection and culture. We then detail procedures for Nanoluciferase mRNA transfection. This protocol facilitates further investigations into the pluripotency and regeneration mechanisms within neoblasts. For complete details on the use and execution of this protocol, please refer to Lei et al.1.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Planarians
Limits:
Animals
Language:
En
Journal:
STAR Protoc
Year:
2024
Document type:
Article
Country of publication: