5- methylcytidine effectively improves spermatogenesis recovery in busulfan-induced oligoasthenospermia mice.
Eur J Pharmacol
; 967: 176405, 2024 Mar 15.
Article
in En
| MEDLINE
| ID: mdl-38341078
ABSTRACT
The function and regulatory mechanisms of 5-methylcytidine (m5C) in oligoasthenospermia remain unclear. In this study, we made a mouse model of oligoasthenospermia through the administration of busulfan (BUS). For the first time, we demonstrated that m5C levels decreased in oligoasthenospermia. The m5C levels were upregulated through the treatments of 5-methylcytidine. The testicular morphology and sperm concentrations were improved via upregulating m5C. The cytoskeletal regenerations of testis and sperm were accompanying with m5C treatments. m5C treatments improved T levels and reduced FSH and LH levels. The levels of ROS and MDA were significantly reduced through m5C treatments. RNA sequencing analysis showed m5C treatments increased the expression of genes involved in spermatid differentiation/development and cilium movement. Immunofluorescent staining demonstrated the regeneration of cilium and quantitative PCR (qPCR) confirmed the high expression of genes involved in spermatogenesis. Collectively, our findings suggest that the upregulation of m5C in oligoasthenospermia facilitates testicular morphology recovery and male infertility via multiple pathways, including cytoskeletal regeneration, hormonal levels, attenuating oxidative stress, spermatid differentiation/development and cilium movement. m5C may be a potential therapeutic agent for oligoasthenospermia.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Semen
/
Busulfan
/
Cytidine
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Eur J Pharmacol
Year:
2024
Document type:
Article
Affiliation country:
Country of publication: