Fluorochrome-labeled inhibitors of caspase-1 require membrane permeabilization to efficiently access caspase-1 in macrophages.

Thygesen, Sara J; Burgener, Sabrina S; Mudai, Prerna; Monteleone, Mercedes; Boucher, Dave; Sagulenko, Vitaliya; Schroder, Kate; Stacey, Katryn J

Thygesen, Sara J; Burgener, Sabrina S; Mudai, Prerna; Monteleone, Mercedes; Boucher, Dave; Sagulenko, Vitaliya; Schroder, Kate; Stacey, Katryn J.

Affiliation

Thygesen SJ; School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Australia.
Burgener SS; Institute for Molecular Bioscience (IMB) and IMB Centre for Inflammation and Disease Research, The University of Queensland, St Lucia, Australia.
Mudai P; School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Australia.
Monteleone M; Institute for Molecular Bioscience (IMB) and IMB Centre for Inflammation and Disease Research, The University of Queensland, St Lucia, Australia.
Boucher D; York Biomedical Research Institute, Department of Biology, University of York, York, United Kingdom.
Sagulenko V; School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Australia.
Schroder K; Institute for Molecular Bioscience (IMB) and IMB Centre for Inflammation and Disease Research, The University of Queensland, St Lucia, Australia.
Stacey KJ; Australian Infectious Diseases Research Centre, The University of Queensland, St Lucia, Australia.

Eur J Immunol ; 54(5): e2350515, 2024 May.

Article in En | MEDLINE | ID: mdl-38361219

ABSTRACT

ABSTRACT

Caspase-1 location in cells has been studied with fluorochrome-labeled inhibitors of caspase-1 (FLICA reagents). We report that FLICA reagents have limited cell-membrane permeability. This impacts experimental design as cells with intact membranes, including caspase-1 knockout cells, are not appropriate controls for cells with inflammasome-induced gasdermin D membrane pores.

Subject(s)
Key words

Caspase inhibitor; Caspase1; FLICA; Gasdermin D; Inflammasome

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Membrane Permeability / Caspase 1 / Inflammasomes / Caspase Inhibitors / Fluorescent Dyes / Macrophages Limits: Animals / Humans Language: En Journal: Eur J Immunol / Eur. j. immunol / European journal of immunology Year: 2024 Document type: Article Affiliation country: Country of publication:

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