Formation of an instantaneous nick for highly efficient adenylation of oligonucleotides by ligase without subsequent jointing.
Chem Commun (Camb)
; 60(21): 2942-2945, 2024 Mar 07.
Article
in En
| MEDLINE
| ID: mdl-38374791
ABSTRACT
By forming a nick at the adenylation site instantaneously, nucleic acids are efficiently adenylated by T4 DNA ligase. The subsequent ligation is successfully suppressed in terms of rapid conversion of the instantaneous nick to a more stable gap. It is helpful to understand enzymatic ligation dynamics, and the adenylated products can be used for various practical applications.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oligonucleotides
/
Ligases
Language:
En
Journal:
Chem Commun (Camb)
Journal subject:
QUIMICA
Year:
2024
Document type:
Article
Country of publication: