Mass spectrometry imaging-based metabolomics highlights spatial metabolic alterations in three types of liver injuries.
J Pharm Biomed Anal
; 242: 116030, 2024 May 15.
Article
in En
| MEDLINE
| ID: mdl-38382318
ABSTRACT
Liver's distinctive function renders it highly susceptible to diverse damage sources. Characterizing the metabolic profiles and spatial signatures in different liver injuries is imperative for early diagnosis and etiology-oriented treatment. In this comparative study, we conducted whole-body spatial metabolomics on zebrafish with liver injury induced by ethanol (EtOH), acetaminophen (APAP), and thioacetamide (TAA). The two specific levels, the whole-body and liver-specific metabolic profiles, as well as their regional distributions, were systematically mapped in situ by mass spectrometry imaging, which is distinct from conventional LC-MS and GC-MS methods. We found that liver injury regions exhibited more pronounced metabolic reprogramming than the entire organism, leading to significant alterations in eight fatty acids, three phospholipids, and four low-molecular-weight metabolites. More importantly, fatty acids as well as small molecule metabolites including glutamine, glutamate, taurine and malic acid displayed contrasting changes between alcoholic liver disease (ALD) and non-alcoholic fatty liver disease (NAFLD). In addition, phospholipids, including Lyso PC (160) and Lyso PE (180), demonstrated notable down-regulation in all damaged liver, whereas PC (341) underwent upregulation. This study not only deepens insights into distinct potential biomarkers for liver injuries, but also underscores spatial metabolomics as a powerful tool to elucidate possible pathogenic mechanisms in other metabolic diseases.
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1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Zebrafish
/
Non-alcoholic Fatty Liver Disease
Limits:
Animals
Language:
En
Journal:
J Pharm Biomed Anal
Year:
2024
Document type:
Article
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