African swine fever virus early protein pI73R suppresses the type-I IFN promoter activities.
Virus Res
; 343: 199342, 2024 05.
Article
in En
| MEDLINE
| ID: mdl-38408646
ABSTRACT
African swine fever virus is known to suppress type-I interferon (IFN) responses. The main objective of this study was to screen early-expressed viral genes for their ability to suppress IFN production. Out of 16 early genes examined, I73R exhibited robust suppression of cGAS-STING-induced IFN-ß promoter activities, impeding the function of both IRF3 and NF-κB transcription factors. As a result, I73R obstructed IRF3 nuclear translocation following the treatment of cells with poly(dAdT), a strong inducer of the cGAS-STING signaling pathway. Although the I73R protein exhibits structural homology with the Zα domain binding to the left-handed helical form of DNA known as Z-DNA, its ability to suppress cGAS-STING induction of IFN-ß was independent of Z-DNA binding activity. Instead, the α3 and ß1 domains of I73R played a significant role in suppressing cGAS-STING induction of IFN-ß. These findings offer insights into the protein's functions and support its role as a virulence factor.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Interferon Type I
/
African Swine Fever
/
African Swine Fever Virus
/
DNA, Z-Form
Limits:
Animals
Language:
En
Journal:
Virus Res
Journal subject:
VIROLOGIA
Year:
2024
Document type:
Article
Affiliation country:
Country of publication: