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Protocol for xenografting of BxPC-3 pancreatic tumors using a chorioallantoic membrane model.
Colenbier, Robin; Coppens, Lenny; Logghe, Tine; van Zwol, Eke; Timmermans, Jean-Pierre; Bogers, Johannes.
Affiliation
  • Colenbier R; Laboratory of Cell Biology and Histology, Faculty of Medicine and Health Sciences, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, 2610 Antwerp, Belgium. Electronic address: robin.colenbier@uantwerpen.be.
  • Coppens L; Laboratory of Cell Biology and Histology, Faculty of Medicine and Health Sciences, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, 2610 Antwerp, Belgium.
  • Logghe T; ElmediX NV, 2800 Mechelen, Belgium.
  • van Zwol E; ElmediX NV, 2800 Mechelen, Belgium.
  • Timmermans JP; Laboratory of Cell Biology and Histology, Faculty of Medicine and Health Sciences, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, 2610 Antwerp, Belgium.
  • Bogers J; Laboratory of Cell Biology and Histology, Faculty of Medicine and Health Sciences, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, 2610 Antwerp, Belgium; ElmediX NV, 2800 Mechelen, Belgium. Electronic address: john-paul.bogers@uantwerpen.be.
STAR Protoc ; 5(2): 102968, 2024 Jun 21.
Article in En | MEDLINE | ID: mdl-38492226
ABSTRACT
The chorioallantoic membrane (CAM) model is an increasingly attractive model for the study of human tumors. However, concise techniques for the use of pancreatic ductal adenocarcinoma BxPC-3 xenografts in CAM assays are not yet available. Here, we present a protocol for the induction of BxPC-3 xenograft tumors with high grafting efficiency. We describe steps for embryo incubation, egg handling, and grafting, each of which has been optimized to prevent fungal contamination and minimize mortality.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pancreatic Neoplasms / Chorioallantoic Membrane Limits: Animals / Humans Language: En Journal: STAR Protoc Year: 2024 Document type: Article Country of publication:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pancreatic Neoplasms / Chorioallantoic Membrane Limits: Animals / Humans Language: En Journal: STAR Protoc Year: 2024 Document type: Article Country of publication: