Dynamic simulation and experimental studies of molecularly imprinted label-free sensor for determination of milk quality marker.
Food Chem
; 449: 139238, 2024 Aug 15.
Article
in En
| MEDLINE
| ID: mdl-38583401
ABSTRACT
Bovine serum albumin (BSA) has emerged as a biomarker for mammary gland health and cow quality, being recognized as a significant allergenic protein. In this study, a novel flexible molecular imprinted electrochemical sensor by surface electropolymerization using pyrrole (Py) as functional monomer, which can be better applied to the detection of milk quality marker BSA. Based on computational results, with regard to all polypyrrole (PPy) conformations and amino-acid positions within the protein, the BSA molecule remained firmly embedded into PPy polymers with no biological changes. The molecular imprinted electrochemical sensor displayed a broad linear detection range from 1.0 × 10-4 to 50 ng·mL-1 (R2 = 0.995) with a low detection limit (LOD) of 4.5 × 10-2 pg·mL-1. Additionally, the sensor was highly selective, reproducible, stable and recoverable, suggesting that it might be utilized for the evaluation of milk quality.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Serum Albumin, Bovine
/
Milk
/
Molecular Imprinting
Limits:
Animals
Language:
En
Journal:
Food Chem
Year:
2024
Document type:
Article
Affiliation country: