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Multi-biomarker approach to evaluate the toxicity of chlorpyrifos (active ingredient and a commercial formulation) on different stages of Biomphalaria straminea.
Bianco, Karina Alesia; Martini, Claudia Noemí; Tejedor, María José; Paredes, María Gimena; Kristoff, Gisela.
Affiliation
  • Bianco KA; Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Laboratorio de Evaluación Ecotoxicológica del Agua Invertebrados Nativos y otros Modelos (EEAINM), Buenos Aires, Argentina; CONICET - Universidad de Buenos Aires, Instituto de Química Biológica
  • Martini CN; Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Laboratorio de Evaluación Ecotoxicológica del Agua Invertebrados Nativos y otros Modelos (EEAINM), Buenos Aires, Argentina; CONICET - Universidad de Buenos Aires, Instituto de Química Biológica
  • Tejedor MJ; Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Laboratorio de Evaluación Ecotoxicológica del Agua Invertebrados Nativos y otros Modelos (EEAINM), Buenos Aires, Argentina.
  • Paredes MG; Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Laboratorio de Evaluación Ecotoxicológica del Agua Invertebrados Nativos y otros Modelos (EEAINM), Buenos Aires, Argentina; CONICET - Universidad de Buenos Aires, Instituto de Química Biológica
  • Kristoff G; Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Laboratorio de Evaluación Ecotoxicológica del Agua Invertebrados Nativos y otros Modelos (EEAINM), Buenos Aires, Argentina; CONICET - Universidad de Buenos Aires, Instituto de Química Biológica
Article in En | MEDLINE | ID: mdl-38615808
ABSTRACT
Biomphalaria straminea is a freshwater gastropod native to South America and used in toxicological assessments. Our aim was to estimate 48 h-LC50 and sub-chronic effects after the exposure to low concentrations of chlorpyrifos as commercial formulation (CF) and active ingredient (AI) on B. straminea adult, embryos and juveniles. Concentrations between 1 and 5000 µg L-1 were chosen for acute exposures and 0.1 and 1 µg L-1 for the sub-chronic one. After 14 days biochemical parameters, viability and sub-populations of hemocytes, reproductive parameters, embryotoxicity and offspring' survival were studied. Egg masses laid between day 12 and 14 were separated to continue the exposure and the embryos were examined daily. Offspring' survival and morphological changes were registered for 14 days after hatching. 48 h-LC50, NOEC and LOEC were similar between CF and AI, however the CF caused more sub-lethal effects. CF but not the AI decreased carboxylesterases, catalase and the proportion of hyalinocytes with respect to the total hemocytes, and increased superoxide dismutase and the % of granulocytes with pseudopods. Also CF caused embryotoxicity probably due to the increase of embryos' membrane permeability. Acetylcholinesterase, superoxide dismutase, hemocytes sub-populations, the time and rate of hatching and juveniles' survival were the most sensitive biomarkers. We emphasize the importance of the assessment of a battery of biomarkers as a useful tool for toxicity studies including reproduction parameters and immunological responses. Also, we highlight the relevance of incorporating the evaluation of formulations in order to not underestimate the effects of pesticides on the environment.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Water Pollutants, Chemical / Biomphalaria / Biomarkers / Chlorpyrifos / Embryo, Nonmammalian / Insecticides Limits: Animals Language: En Journal: Comp Biochem Physiol C Toxicol Pharmacol Journal subject: FARMACOLOGIA / TOXICOLOGIA Year: 2024 Document type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Water Pollutants, Chemical / Biomphalaria / Biomarkers / Chlorpyrifos / Embryo, Nonmammalian / Insecticides Limits: Animals Language: En Journal: Comp Biochem Physiol C Toxicol Pharmacol Journal subject: FARMACOLOGIA / TOXICOLOGIA Year: 2024 Document type: Article