Generation of a PPM1A-deficient human induced pluripotent stem cell line using CRISPR-Cas9 technology.
Stem Cell Res
; 77: 103420, 2024 Jun.
Article
in En
| MEDLINE
| ID: mdl-38643711
ABSTRACT
PPM1A is a member of the serine/threonine protein phosphatase family. It can bind to a variety of proteins to dephosphorylate them, and extensively regulates many life activities such as cell growth, cell stress, immune response, and tumor formation. Here we constructed a human induced pluripotent stem cell (hiPSC) line with knockout of PPM1A using CRISPR/Cas9-mediated gene targeting. This cell line exhibits normal karyotype, pluripotency, and trilineage differentiation potential, which could provide a useful cellular resource for exploring the mechanism of PPM1A in regulating downstream signaling pathways and explore the application of PPM1A in anti-tumor and anti-infection.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Induced Pluripotent Stem Cells
/
CRISPR-Cas Systems
/
Protein Phosphatase 2C
Limits:
Humans
Language:
En
Journal:
Stem Cell Res
Year:
2024
Document type:
Article