L-Glutamine Substantially Improves 5-Fluorouracil-Induced Intestinal Mucositis by Modulating Gut Microbiota and Maintaining the Integrity of the Gut Barrier in Mice.
Mol Nutr Food Res
; 68(9): e2300704, 2024 May.
Article
in En
| MEDLINE
| ID: mdl-38656560
ABSTRACT
SCOPE This study investigates the potential of glutamine to mitigate intestinal mucositis and dysbiosis caused by the chemotherapeutic agent 5-fluorouracil (5-FU). METHODS AND RESULTS:
Over twelve days, Institute of Cancer Research (ICR) mice are given low (0.5 mg kg-1) or high (2 mg kg-1) doses of L-Glutamine daily, with 5-FU (50 mg kg-1) administered between days six and nine. Mice receiving only 5-FU exhibited weight loss, diarrhea, abnormal cell growth, and colonic inflammation, correlated with decreased mucin proteins, increased endotoxins, reduced fecal short-chain fatty acids, and altered gut microbiota. Glutamine supplementation counteracted these effects by inhibiting the Toll-like receptor 4/nuclear factor kappa B (TLR4/NF-κB) pathway, modulating nuclear factor erythroid 2-related factor 2/heme oxygenase 1 (Nrf2/HO-1) oxidative stress proteins, and increasing mammalian target of rapamycin (mTOR) levels, thereby enhancing microbial diversity and protecting intestinal mucosa.CONCLUSIONS:
These findings underscore glutamine's potential in preventing 5-FU-induced mucositis by modulating gut microbiota and inflammation pathways.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Mucositis
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Fluorouracil
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Gastrointestinal Microbiome
/
Glutamine
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Intestinal Mucosa
Limits:
Animals
Language:
En
Journal:
Mol Nutr Food Res
Journal subject:
CIENCIAS DA NUTRICAO
Year:
2024
Document type:
Article
Affiliation country: