Frequency shift Raman-based sensing of serum MicroRNA for ultrasensitive cervical cancer diagnosis.
Photodiagnosis Photodyn Ther
; 46: 104105, 2024 Apr.
Article
in En
| MEDLINE
| ID: mdl-38677498
ABSTRACT
Cervical cancer is the most common gynaecological tumor. The development of a sensor for the ultrasensitive detection of cervical cancer is significant in guaranteeing its prognosis. Herein, we proposed a novel surface-enhanced Raman scattering (SERS) analysis platform using a frequency shifts-based sensing model for rapid and ultrasensitive microRNA (miRNA) assay. During the analysis process, miR-21 can be captured by the single-stranded DNA (ssDNA) modified on the platform which is complementary pairing with miR-21. The connection of miR-21 can lead to the variation of the molecular weight and result in the deformation extent of the Raman report molecule 6Thioguanine (6TG); thus, the peak at 1301 cm-1 due to the ring C-N stretches of 6TG shifts to lower frequency. The detection limit (LOD) of the proposed SERS analysis platform is as low as 8.32 aM. Moreover, the platform also has excellent specificity and repeatability, with the relative standard deviation (RSD) value of 6.53 %. Serum samples of cervical cancer patients and healthy subjects were analyzed via the platform and the accuracy of the detection results was verified by qRT-PCR, revealing that SERS results and qRT-PCR results have high homogeneity. Thus, the platform can serve as a potential tool for clinical diagnosis of cervical cancer.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spectrum Analysis, Raman
/
Uterine Cervical Neoplasms
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MicroRNAs
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Limit of Detection
Limits:
Female
/
Humans
Language:
En
Journal:
Photodiagnosis Photodyn Ther
Journal subject:
DIAGNOSTICO POR IMAGEM
/
TERAPEUTICA
Year:
2024
Document type:
Article
Affiliation country:
Country of publication: