PagWOX11/12a from hybrid poplar enhances drought tolerance by modulating reactive oxygen species.
Plant Physiol Biochem
; 211: 108662, 2024 Jun.
Article
in En
| MEDLINE
| ID: mdl-38691876
ABSTRACT
WOX11/12 is a homeobox gene of WOX11 and WOX12 in Arabidopsis that plays important roles in crown root development and growth. It has been reported that WOX11/12 participates in adventitious root (AR) formation and different abiotic stress responses, but the downstream regulatory network of WOX11/12 in poplar remains to be further investigated. In this study, we found that PagWOX11/12a is strongly induced by PEG-simulated drought stress. PagWOX11/12a-overexpressing poplar plantlets showed lower oxidative damage levels, greater antioxidant enzyme activities and reactive oxygen species (ROS) scavenging capacity than non-transgenic poplar plants, whereas PagWOX11/12a dominant repression weakened root biomass accumulation and drought tolerance in poplar. RNA-seq analysis revealed that several differentially expressed genes (DEGs) regulated by PagWOX11/12a are involved in redox metabolism and drought stress response. We used RT-qPCR and yeast one-hybrid (Y1H) assays to validate the downstream target genes of PagWOX11/12a. These results provide new insights into the biological function and molecular regulatory mechanism of WOX11/12 in the abiotic resistance processes of poplar.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Proteins
/
Reactive Oxygen Species
/
Gene Expression Regulation, Plant
/
Populus
/
Droughts
Language:
En
Journal:
Plant Physiol Biochem
Journal subject:
BIOQUIMICA
/
BOTANICA
Year:
2024
Document type:
Article
Affiliation country:
Country of publication: