Anoctamin-like protein 1 regulates repolarization in Paramecium behavioral responses.
J Eukaryot Microbiol
; 71(4): e13030, 2024.
Article
in En
| MEDLINE
| ID: mdl-38757880
ABSTRACT
Paramecium exhibits responsive behavior to environmental changes, moving either closer to or further away from stimuli. Electrophysiological experiments have revealed that these behavioral responses are controlled by membrane potentials. Anoctamin, a Ca2+-activated Cl- channel, is involved in the regulation of membrane potential in mammals. However, it remains uncertain whether Cl- channels like anoctamin regulate Paramecium behavior. Herein, replacement of external Cl- ions with acetate ion and application of Cl- channel blocker niflumic acid (NFA, 0.1 µM) increased spontaneous avoiding reactions (sARs). Hence, we hypothesized that anoctamin is involved in the stabilization of membrane potential fluctuation. Paramecium cells in which the anoctamin-like protein 1 gene was knocked down displayed frequent sARs in the culture medium without external stimulation. Treatment of anoctamin-like protein 1-knockdown cells with the Ca2+ chelator BAPTA or Ca-channel blocker nicardipine reversed the increase in sARs. Electrophysiological experiments revealed extension of membrane depolarization when positive currents were applied to anoctamin-like protein 1-knockdown cells. We concluded that anoctamin-like protein 1 works as a Cl-channel and stabilizes the membrane potential oscillation, reducing sARs.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Paramecium
/
Protozoan Proteins
/
Membrane Potentials
Language:
En
Journal:
J Eukaryot Microbiol
Journal subject:
MICROBIOLOGIA
/
PARASITOLOGIA
Year:
2024
Document type:
Article
Affiliation country:
Country of publication: