Probing the Surface Layer Modulation on Archaeal Mechanics and Adhesion at the Single-Cell Level.
Anal Chem
; 96(22): 8981-8989, 2024 06 04.
Article
in En
| MEDLINE
| ID: mdl-38758609
ABSTRACT
Addressing the challenge of understanding how cellular interfaces dictate the mechanical resilience and adhesion of archaeal cells, this study demonstrates the role of the surface layer (S-layer) in methanogenic archaea. Using a combination of atomic force microscopy and single-cell force spectroscopy, we quantified the impact of S-layer disruption on cell morphology, mechanical properties, and adhesion capabilities. We demonstrate that the S-layer is crucial for maintaining cell morphology, where its removal induces significant cellular enlargement and deformation. Mechanical stability of the cell surface is substantially compromised upon S-layer disruption, as evidenced by decreased Young's modulus values. Adhesion experiments revealed that the S-layer primarily facilitates hydrophobic interactions, which are significantly reduced after its removal, affecting both cell-cell and cell-bubble interactions. Our findings illuminate the S-layer's fundamental role in methanogen architecture and provide a chemical understanding of archaeal cell surfaces, with implications for enhancing methane production in biotechnological applications.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Surface Properties
/
Microscopy, Atomic Force
/
Single-Cell Analysis
Language:
En
Journal:
Anal Chem
Year:
2024
Document type:
Article