Saururus chinensis (Lour.) Baill. extract promotes skeletal muscle cell differentiation by positively regulating mitochondrial biogenesis and AKT/mTOR signaling in vitro.
Mol Med Rep
; 30(1)2024 07.
Article
in En
| MEDLINE
| ID: mdl-38785149
ABSTRACT
Promotion of myoblast differentiation by activating mitochondrial biogenesis and protein synthesis signaling pathways provides a potential alternative strategy to balance energy and overcome muscle loss and muscle disorders. Saururus chinensis (Lour.) Baill. extract (SCE) has been used extensively as a traditional herbal medicine and has several physiological activities, including antiasthmatic, antioxidant, antiinflammatory, antiatopic, anticancer and hepatoprotective properties. However, the effects and mechanisms of action of SCE on muscle differentiation have not yet been clarified. In the present study, it was investigated whether SCE affects skeletal muscle cell differentiation through the regulation of mitochondrial biogenesis and protein synthesis in murine C2C12 myoblasts. The XTT colorimetric assay was used to determine cell viability, and myosin heavy chain (MyHC) levels were determined using immunocytochemistry. SCE was applied to C2C12 myotube at different concentrations (1, 5, or 10 ng/ml) and times (1,3, or 5 days). Reverse transcriptionquantitative PCR and western blotting were used to analyze the mRNA and protein expression change of factors related to differentiation, mitochondrial biogenesis and protein synthesis. Treatment of C2C12 cells with SCE at 1,5, and 10 ng/ml did not affect cell viability. SCE promoted C2C12 myotube formation and significantly increased MyHC expression in a concentration and timedependent manner. SCE significantly increased the mRNA and protein expression of muscle differentiationspecific markers, such as MyHC, myogenic differentiation 1, myogenin, Myogenic Factor 5, and ßcatenin, mitochondrial biosynthesisrelated factors, such as peroxisome proliferatoractivated receptorgamma coactivator1α, nuclear respirator factor1, AMPactivated protein kinase phosphorylation, and histone deacetylase 5 and AKT/mTOR signaling factors related to protein synthesis. SCE may prevent skeletal muscle dysfunction by enhancing myoblast differentiation through the promotion of mitochondrial biogenesis and protein synthesis.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Organelle Biogenesis
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Plant Extracts
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Signal Transduction
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Cell Differentiation
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Saururaceae
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Proto-Oncogene Proteins c-akt
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TOR Serine-Threonine Kinases
Limits:
Animals
Language:
En
Journal:
Mol Med Rep
Year:
2024
Document type:
Article