Protocol for identifying OXCT1-mediated LACTB succinylation sites in vitro.
STAR Protoc
; 5(2): 103088, 2024 Jun 21.
Article
in En
| MEDLINE
| ID: mdl-38787728
ABSTRACT
OXCT1 acts as a succinyltransferase to promote serine beta-lactamase-like protein (LACTB) K284 succinylation. Here, we present a protocol for detecting OXCT1-mediated LACTB succinylation levels and sites. We describe steps for using western blotting (WB) and mass spectrometry to determine OXCT1-mediated LACTB succinylation levels and sites in vitro. This protocol can be applied to detect and identify succinylation levels and sites on other proteins. For complete details on the use and execution of this protocol, please refer to Ma et al.1.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Beta-Lactamases
Language:
En
Journal:
STAR Protoc
Year:
2024
Document type:
Article
Affiliation country:
Country of publication: