Sesame oil as a partial substitute for egg yolk in goat semen extenders.
Anim Reprod Sci
; 266: 107500, 2024 Jul.
Article
in En
| MEDLINE
| ID: mdl-38820784
ABSTRACT
This study aimed to evaluate the effects of replacing egg yolk extender with sesame oil on the quality of sperm in goats following incubation at 37°C, chilling at 4°C, and freezing. Semen samples were collected from four intact male goats. The individual semen sample was divided into six groups consisting of a control group and five treatment groups with different egg yolk-to-sesame oil ratios. Seminal plasma was removed, and the sperm pellet was diluted with experimental semen extenders. The control group contained an extender of 10â¯% egg yolk (SO0), and the experimental extenders were composed of 8.75â¯% egg yolk and 1.25â¯% sesame oil (SO1.25); 7.5â¯% egg yolk and 2.5â¯% sesame oil (SO2.5); 5â¯% egg yolk and 5â¯% sesame oil (SO5); 2.5â¯% egg yolk and 7.5â¯% sesame oil (SO7.5); and 10â¯% sesame oil (SO10). Each group of semen was divided into three groups, incubated at 37°C for 1â¯h, chilled at 4°C for 4â¯h, or frozen for 24â¯h. Five replicates were performed. Sperm quality was evaluated, including motility, viability, and functional membrane integrity. The SO1.25 group achieved the highest sperm quality rate among the treatment groups, and the extender did not have a negative effect compared to the control. However, the total replacement of egg yolk with sesame oil in an extender resulted in the lowest sperm quality. In conclusion, the ratios of egg yolk and sesame oil that were acceptable for goat semen cryopreservation were 8.75â¯% and 1.25â¯%, respectively.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Semen Preservation
/
Goats
/
Sesame Oil
/
Cryopreservation
/
Egg Yolk
/
Semen Analysis
Limits:
Animals
Language:
En
Journal:
Anim Reprod Sci
Year:
2024
Document type:
Article