CdS/Bi2S3/NiS ternary heterostructure-based photoelectrochemical immunosensor for the sensitive detection of carbohydrate antigen 125.
Anal Chim Acta
; 1312: 342765, 2024 Jul 11.
Article
in En
| MEDLINE
| ID: mdl-38834279
ABSTRACT
The sensitive, accurate and rapid detection of carbohydrate antigen 125 (CA125) is essential for the early diagnosis and clinical management of ovarian cancer, but there is still challenge. Herein, a photoelectrochemical (PEC) immunosensor based on CdS/Bi2S3/NiS ternary sulfide heterostructured photocatalyst was presented for the detection of CA125. The CdS/Bi2S3/NiS was synthesized by a one-step hydrothermal approach. The heterojunction comprising of CdS and Bi2S3 could separate photogenerated carriers, the introduced narrow bandgap NiS could act as electron-conducting bridge to facilitate the transfer of interfacial photogenerated electrons, thereby improving the photoelectric conversion efficiency. Due to their synergistic effect, the photocurrent response produced by the composite was up to 14.6 times of pure CdS. On the basis, a PEC immunosensor was constructed by introducing the CA125 antibody through thioglycolic acid linkage. It was found that the resulting immunosensor showed good performance. Under the optimized conditions, its linear detection range was as wide as 1 pg mL-1-50 ng mL-1, and the detection limit was low to 0.85 pg mL-1. Furthermore, we experimentally tested its anti-interference, stability and reproducibility, and satisfactory results were achieved. The practicable feasibility of the sensor was confirmed by testing serum sample. Thus this work provided a simple, fast and enough sensitive approach for CA125 monitoring.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sulfides
/
Bismuth
/
CA-125 Antigen
/
Cadmium Compounds
/
Electrochemical Techniques
Limits:
Humans
Language:
En
Journal:
Anal Chim Acta
Year:
2024
Document type:
Article