Your browser doesn't support javascript.
loading
Antimicrobial susceptibility of clinical Helicobacter pylori isolates and its eradication by standard triple therapy: a study in west central region of Colombia.
Alvarez-Aldana, Adalucy; Fernandez Uribe, Paula Andrea; Mejía Valencia, Tatiana; Guaca-Gonzalez, Yina Marcela; Santacruz-Ibarra, Jorge Javier; Arturo-Arias, Brenda Lucia; Castañeda-Chavez, Luis Javier; Pacheco-López, Robinson; Londoño-Giraldo, Lina María; Moncayo-Ortiz, José Ignacio.
Affiliation
  • Alvarez-Aldana A; Grupo de Investigación en Microbiología y Biotecnología (MICROBIOTEC), Universidad Libre Seccional Pereira, Pereira, Colombia.
  • Fernandez Uribe PA; Grupo de Investigación ESCULAPIO, Universidad Libre Seccional Cali, Cali, Colombia.
  • Mejía Valencia T; Grupo de Investigación en Gerencia del Cuidado, Universidad Libre Seccional Pereira, Pereira, Colombia.
  • Guaca-Gonzalez YM; Grupo de Investigación en Enfermedades Infecciosas (GRIENI), Universidad Tecnológica de Pereira, Pereira, Colombia.
  • Santacruz-Ibarra JJ; Grupo de Investigación en Enfermedades Infecciosas (GRIENI), Universidad Tecnológica de Pereira, Pereira, Colombia.
  • Arturo-Arias BL; Grupo de Investigación Médica, Universidad de Manizales, Manizales, Colombia.
  • Castañeda-Chavez LJ; SES Hospital Universitario de Caldas, Manizales, Colombia.
  • Pacheco-López R; Centro de Especialistas de Risaralda, Pereira, Colombia.
  • Londoño-Giraldo LM; Grupo de Investigación ESCULAPIO, Universidad Libre Seccional Cali, Cali, Colombia.
  • Moncayo-Ortiz JI; Grupo de Investigación en Microbiología y Biotecnología (MICROBIOTEC), Universidad Libre Seccional Pereira, Pereira, Colombia.
Microbiol Spectr ; 12(8): e0040124, 2024 Aug 06.
Article in En | MEDLINE | ID: mdl-38916348
ABSTRACT
The aim of the present study was first to isolate Helicobacter pylori from gastric biopsy specimens and to test their antibiotic susceptibility. Second, it was to evaluate the efficacy of the standard triple therapy from patients of the west central region of Colombia. H. pylori positive patients received standard triple therapy with proton pump inhibitor (PPI) (40 mg b.i.d.), clarithromycin (500 mg b.i.d.), and amoxicillin (1 g b.i.d.) for 14 days. Thereafter, antibiotic susceptibility of the isolates was assessed by E-Test. From 94 patients enrolled, 67 were positive for H. pylori by histology or culture. Overall resistance to metronidazole, levofloxacin, rifampicin, clarithromycin, and amoxicillin was 81%, 26.2%, 23.9%, 19%, and 9.5%, respectively. No resistance was found for tetracycline. A total of 54 patients received standard triple therapy, 48 attended follow-ups testing, and of them, 30 had resistance test reports. Overall eradication rate was 81.2%. Second-line treatment was given to eight patients, four of whom were followed up with a 13C urea breath test (UBT) and remained positive for H. pylori. Eradication was significantly higher in patients with clarithromycin susceptible than in resistant strains (95.6% vs 42.8% P = 0.001). The updated percentages of resistance to clarithromycin in this geographical area had increased, so this value must be considered when choosing the treatment regimen.IMPORTANCEAntibiotic resistance in Helicobacter pylori has increased worldwide, as has resistance to multiple antimicrobials (MDRs), which seriously hampers the successful eradication of the infection. The ideal success rate in eradicating H. pylori infection (≥90%) was not achieved in this study (81.2%). This is the first time that MDR is reported (14.3%) in the region; the resistance to clarithromycin increased over time (3.8%-19%), and levofloxacin (26.2%) and rifampicin (23%) resistant isolates were detected for the first time. With these results, strain susceptibility testing is increasingly important, and the selection of treatment regimen should be based on local antibiotic resistance patterns.
Subject(s)
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Microbial Sensitivity Tests / Helicobacter pylori / Helicobacter Infections / Clarithromycin / Drug Therapy, Combination / Amoxicillin / Anti-Bacterial Agents Limits: Adult / Aged / Female / Humans / Male / Middle aged Country/Region as subject: America do sul / Colombia Language: En Journal: Microbiol Spectr Year: 2024 Document type: Article Affiliation country:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Microbial Sensitivity Tests / Helicobacter pylori / Helicobacter Infections / Clarithromycin / Drug Therapy, Combination / Amoxicillin / Anti-Bacterial Agents Limits: Adult / Aged / Female / Humans / Male / Middle aged Country/Region as subject: America do sul / Colombia Language: En Journal: Microbiol Spectr Year: 2024 Document type: Article Affiliation country: