Enhancing the substrate selectivity of enzyme mimetics in biosensing and bioassay: Novel approaches.
Adv Colloid Interface Sci
; 331: 103233, 2024 Sep.
Article
in En
| MEDLINE
| ID: mdl-38924801
ABSTRACT
A substantial development in nanoscale materials possessing catalytic activities comparable with natural enzymes has been accomplished. Their advantages were owing to the excellent sturdiness in an extreme environment, possibilities of their large-scale production resulting in higher profitability, and easy manipulation for modification. Despite these advantages, the main challenge for artificial enzyme mimetics is the lack of substrate selectivity where natural enzymes flourish. This review addresses this vital problem by introducing substrate selectivity strategies to three classes of artificial enzymes molecularly imprinted polymers, nanozymes (NZs), and DNAzymes. These rationally designed strategies enhance the substrate selectivity and are discussed and exemplified throughout the review. Various functional mechanisms associated with applying enzyme mimetics in biosensing and bioassays are also given. Eventually, future directives toward enhancing the substrate selectivity of biomimetics and related challenges are discussed and evaluated based on their efficiency and convenience in biosensing and bioassays.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biological Assay
/
Biosensing Techniques
/
Biomimetic Materials
Limits:
Humans
Language:
En
Journal:
Adv Colloid Interface Sci
/
Adv. colloid interface sci
/
Advances in colloid and interface science
Journal subject:
QUIMICA
Year:
2024
Document type:
Article
Country of publication: