A change in metal cation switches selectivity of a phospholipid sensor from phosphatidic acid to phosphatidylserine.
Org Biomol Chem
; 22(28): 5843-5849, 2024 07 17.
Article
in En
| MEDLINE
| ID: mdl-38957899
ABSTRACT
Phosphatidic acid and phosphatidylserine are anionic phospholipids with emerging signalling roles in cells. Determination of how phosphatidic acid and phosphatidylserine change location and quantity in cells over time requires selective fluorescent sensors that can distinguish these two anionic phospholipids. However, the design of such synthetic sensors that can selectively bind and respond to a single phospholipid within the complex membrane milieu remains challenging. In this work, we present a simple and robust strategy to control the selectivity of synthetic sensors for phosphatidic acid and phosphatidylserine. By changing the coordination metal of a dipicolylamine (DPA) ligand from Zn(II) to Ni(II) on the same synthetic sensor with a peptide backbone, we achieve a complete switch in selectivity from phosphatidic acid to phosphatidylserine in model lipid membranes. Furthermore, this strategy was largely unaffected by the choice and the position of the fluorophores. We envision that this strategy will provide a platform for the rational design of targeted synthetic phospholipid sensors to probe plasma and intracellular membranes.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phosphatidic Acids
/
Phosphatidylserines
/
Picolinic Acids
/
Zinc
/
Fluorescent Dyes
Language:
En
Journal:
Org Biomol Chem
Journal subject:
BIOQUIMICA
/
QUIMICA
Year:
2024
Document type:
Article
Affiliation country: