Structural characteristics of sulfated xylogalactomannan isolated from Caulerpa okamurae and its anticoagulant activity.

ABSTRACT

Due to wonderful taste, rich nutrition and biological functions, many marine green algae in the genus Caulerpa have been recently developed as candidates for green caviar. A novel water-soluble sulfated xylogalactomannan CO-0-1 was obtained from the green algae Caulerpa okamurae. CO-0-1 was mainly composed of mannose (Man), galactose (Gal), and xylose (Xyl) at the ratio of 4.44.01.4 with the molecular weight at 470 kDa and the sulfate content at 12.78 %. The sulfated xylogalactomannan had Man at the backbone with →4)-ß-D-Manp-(1→ and →2)-ß-D-Manp-(1→ as the main chain and branches at O-3 position. The side chains contained →3)-ß-D-Galp-(1→ and minor →2)-ß-D-Xylp(1→. The sulfate groups only distributed at the side chains and at O-6 position of →3)-ß-D-Galp-(1→ and O-4 position of (1→2)-ß-D-Xylp. The anticoagulant activity indicated that CO-0-1 displayed intrinsic anticoagulant and specific anti-thrombin activities. The investigation expanded the utilization and development scene and scope of the green algae Caulerpa okamurae.