Your browser doesn't support javascript.
loading
Meta-analysis of the accuracy for RASSF1A methylation in bronchial aspirates for the diagnosis of lung cancer.
Chen, Xu-Ping; He, Shi-Xu; Chen, Meng-You; Chen, Fu-Bin; Wu, Peng; Shi, Ping; Zhao, Shi-Cai; Zhao, Ling-Yan; Xiong, Xiao-Min; Zeng, Jia.
Affiliation
  • Chen XP; Department of Respiratory and Critical Care Medicine, Guangyuan Central Hospital, Guangyuan, Sichuan Province, China.
  • He SX; Department of Respiratory and Critical Care Medicine, Guangyuan Central Hospital, Guangyuan, Sichuan Province, China.
  • Chen MY; Intern, Qiqihar Medical University, Qiqihar, Heilongjiang Province, China.
  • Chen FB; Department of General Internal Medicine, Qianwei Xuefu Community Health Service Center, Leshan, SiChuan Province, China.
  • Wu P; Department of Respiratory and Critical Care Medicine, Guangyuan Central Hospital, Guangyuan, Sichuan Province, China.
  • Shi P; Department of Respiratory and Critical Care Medicine, Guangyuan Central Hospital, Guangyuan, Sichuan Province, China.
  • Zhao SC; Department of Respiratory and Critical Care Medicine, Guangyuan Central Hospital, Guangyuan, Sichuan Province, China.
  • Zhao LY; Department of Respiratory and Critical Care Medicine, Guangyuan Central Hospital, Guangyuan, Sichuan Province, China.
  • Xiong XM; Department of Respiratory and Critical Care Medicine, Guangyuan Central Hospital, Guangyuan, Sichuan Province, China.
  • Zeng J; Department of Respiratory and Critical Care Medicine, Guangyuan Central Hospital, Guangyuan, Sichuan Province, China.
PLoS One ; 19(7): e0299447, 2024.
Article in En | MEDLINE | ID: mdl-39052646
ABSTRACT

OBJECTIVE:

To establish the diagnostic accuracy of RASSF1A (Ras association domain family 1 isoform) methylation using bronchial aspirates as an auxiliary method for diagnosing lung cancer through a systematic review and meta-analysis.

METHODS:

Studies published prior to October 30, 2022, were retrieved from the Embase, PubMed, Web of Science, and Wan Fang databases using the keywords "lung cancer", "RASSF1A", "methylation", and "bronchial aspirates". A fixed or random effect model was used to calculate the combined sensitivity, specificity, positive likelihood ratios (LR), negative LR, diagnostic odds ratio (DOR), along with the respective 95% confidence intervals (CIs) and the area under the curve (AUC) with Q index. The threshold effect was defined by using the Spearman correlation coefficient, and the Deeks funnel plot was generated to evaluate publication bias.

RESULTS:

Among the 12 trials that met the inclusion criteria, a total of 2388 participants were involved. The pooled results for the diagnosis of lung cancer were as follows, when compared to the pathological diagnosis sensitivity of 0.47 (95% CI 0.45-0.50), specificity of 0.96 (95% CI 0.95-0.97), positive LR of 12.18 (95% CI 8.96-16.55), negative LR of 0.56 (95% CI 0.52-0.61), DOR of 24.05 (95% CI 17.29-33.47), and AUC of 0.78 (Q index = 0.72), respectively. The sensitivity of the RASSF1A methylation assay was relatively low in a detailed subgroup analysis, fluctuating between 0.39 and 0.90, indicating a limitation in its diagnostic value for lung cancer. The RASSF1A methylation assay, on the other hand, demonstrated excellent specificity, suggesting a high exclusion value. Of note, the diagnostic sensitivity, specificity, DOR, and AUC for small cell lung cancer were 0.90 (0.84-0.94), 0.95 (0.94-0.97), 249.5 (103.94-598.8), and 0.98, respectively, showing that RASSF1A methylation was a promising biomarker for diagnosing small cell lung cancer with both high diagnostic and exclusion value. Furthermore, RASSF1A methylation using bronchial washings and bronchial aspirates showed a high AUC of 0.998 and 0.93, respectively, indicating excellent diagnostic performance.

CONCLUSIONS:

The methylation of RASSF1A in bronchial aspirates demonstrated a high level of diagnostic accuracy and has the potential to be a valuable supplementary diagnostic method, especially for identifying small cell lung cancer.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA Methylation / Tumor Suppressor Proteins / Lung Neoplasms Limits: Humans Language: En Journal: PLoS One Journal subject: CIENCIA / MEDICINA Year: 2024 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA Methylation / Tumor Suppressor Proteins / Lung Neoplasms Limits: Humans Language: En Journal: PLoS One Journal subject: CIENCIA / MEDICINA Year: 2024 Document type: Article Affiliation country: Country of publication: