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Synthesis, Characterization, and In-Vitro Evaluation of Silibinin-loaded PEGylated Niosomal Nanoparticles: Potential Anti-Cancer Effects on SW480 Colon Cancer Cells.
Alali, Urjwan; Mohammed Kadhim Al-Tu'ma, Maha; Fadhil Jawad, Ammar; Talib Ahmed, Saja; Addai Ali, Hanaa; Abdulzehra, Siham; Jawad Al-Tu'ma, Fadhil.
Affiliation
  • Alali U; Department of Pharmaceutical Chemistry, College of Pharmacy, University of Kerbala, Kerbala, Iraq.
  • Mohammed Kadhim Al-Tu'ma M; Department of Anesthesia Techniques, College of Health and Medical Techniques, Al-Zahraa University for Women, Kerbala, Iraq.
  • Fadhil Jawad A; Department of Pharmacognesy, College of Pharmacy, University of Kerbala, Kerbala, Iraq.
  • Talib Ahmed S; Department of Chemistry, College of Science, University of Kufa, Kufa, Iraq.
  • Addai Ali H; Department of Chemistry, College of Science, University of Kufa, Kufa, Iraq.
  • Abdulzehra S; Department of Clinical Biochemistry and Laboratory Medicine, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Jawad Al-Tu'ma F; Department of Chemistry and Biochemistry, College of Medicine, University of Kerbala, Kerbala, Iraq.
Asian Pac J Cancer Prev ; 25(7): 2539-2550, 2024 Jul 01.
Article in En | MEDLINE | ID: mdl-39068589
ABSTRACT

OBJECTIVE:

Colorectal cancer is a significant global health concern with high mortality rates. Silibinin is a compound derived from milk thistle with anticancer properties and may be a potential treatment option for colorectal cancer. Its poor solubility limits its clinical application, but various strategies, such as nanoparticle encapsulation, have shown promise. In this study, a PEGylated niosomal drug delivery system was used to enhance the solubility of silibinin, and its anti-proliferative effects were evaluated against human colorectal cancer cell lines.

METHODS:

The silibinin-loaded PEGylated niosomal nanoparticles (NIO-SIL) were fabricated using the thin-film hydration method and characterized with dialysis bag, AFM, SEM, DLS, and FTIR systems. Finally, the cancerous cells and human normal cells were treated with NIO-SIL and pure silibinin. The proliferation, apoptosis, and cell cycle of these cells were evaluated. Subsequently, the expression of Bax, Bcl-2, p53, and cyclin D1 genes was measured using real-time PCR.

RESULT:

The drug release profile, size, morphology, and chemical interactions of the synthesized PEGylated niosomal nanoparticles were suitable for use as a drug delivery system. Both pure silibinin and NIO-SIL could reduce the proliferation of cancerous cells, induce apoptosis, and cause cell cycle arrest, with no significant negative effects reported on human normal cells. Both pure silibinin and NIO-SIL reduced the expression of the Bcl-2 and cyclin D1 genes while increasing the expression of Bax and p53. (p-value < 0.05 *).

CONCLUSION:

The outcomes of this study indicate the high potential of PEGylated niosomal nanoparticles for encapsulation and delivery of silibinin to cancer cells, with no negative effects on normal cells.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polyethylene Glycols / Apoptosis / Cell Proliferation / Nanoparticles / Silybin Limits: Humans Language: En Journal: Asian Pac J Cancer Prev Journal subject: NEOPLASIAS Year: 2024 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polyethylene Glycols / Apoptosis / Cell Proliferation / Nanoparticles / Silybin Limits: Humans Language: En Journal: Asian Pac J Cancer Prev Journal subject: NEOPLASIAS Year: 2024 Document type: Article Affiliation country: Country of publication: