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(S)-3-(3,4-Dihydroxybenzyl) piperazine-2,5-dione (cyclo-Gly-L-DOPA or CG-Nio-CGLD) peptide loaded in Chitosan Glutamate-Coated Niosomes as anti-Colorectal cancer activity.
Piri-Gharaghie, Tohid; Ghourchian, Hedieh; Rezaeizadeh, Golnoosh; Kabiri, Hamidreza; Rajaei, Negin; Dhiaa, Aya Mohammed; Ghajari, Ghazal; Bahari, Roghayeh.
Affiliation
  • Piri-Gharaghie T; Biotechnology Research Center, Faculty of Biological Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran. tohidpirie@yahoo.com.
  • Ghourchian H; Department of Biology, Faculty of Biological Science, Tehran North Branch, Islamic Azad University, Tehran, Iran.
  • Rezaeizadeh G; Department of Microbiology, Faculty of Biological Sciences, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
  • Kabiri H; Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
  • Rajaei N; Sina Borna Aria (SABA) Co., Ltd, Research and Development Center for Biotechnology, Shahrekord, Iran.
  • Dhiaa AM; Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
  • Ghajari G; Sina Borna Aria (SABA) Co., Ltd, Research and Development Center for Biotechnology, Shahrekord, Iran.
  • Bahari R; Department of Pharmacy, Al-Noor University College, Nineveh, Iraq.
BMC Pharmacol Toxicol ; 25(1): 44, 2024 Aug 01.
Article in En | MEDLINE | ID: mdl-39090674
ABSTRACT

BACKGROUND:

Colorectal cancer (CRC), now the second most prevalent malignant tumor worldwide, is more prevalent in young adults. In recent decades, there has been progress in creating anti-colorectal cancer medications, including cytotoxic compounds.

OBJECTIVES:

Novel anticancer drugs are needed to surmount existing obstacles. A recent study investigated the effectiveness of novel formulations in preventing colorectal cancer.

METHODS:

During this study, we assessed a new kind of niosome called cyclo-Gly-L-DOPA (CG-Nio-CGLD) made from chitosan glutamate. We evaluated the anti-colorectal cancer properties of CG-Nio-CGLD utilizing CCK-8, invasion assay, MTT assay, flow cytometry, and cell cycle analysis. The transcription of genes associated with apoptosis was analyzed using quantitative real-time PCR. At the same time, the cytotoxicity of nanomaterials on both cancer and normal cell lines was assessed using MTT assays. Novel anticancer drugs are needed to surmount existing obstacles. A recent study investigated the effectiveness of newly developed formulations in preventing colorectal cancer.

RESULTS:

The Nio-CGLD and CG-Nio-CGLD were spherical mean diameters of 169.12 ± 1.87 and 179.26 ± 2.17 nm, respectively. Entrapment efficiency (EE%) measurements of the Nio-CGLD and CG-Nio-CGLD were 63.12 ± 0.51 and 76.43 ± 0.34%, respectively. In the CG-Nio-CGLD group, the percentages of early, late, necrotic, and viable CL40 cells were 341.93%, 23.27%, 9.32%, and 25.48%. The transcription of the genes PP53, cas3, and cas8 was noticeably higher in the treatment group compared to the control group (P > 0.001). Additionally, the treatment group had lower BCL2 and survivin gene expression levels than the control group (P < 0.01). Additionally, CG-Nio-CGLD formulations demonstrated a biocompatible nanoscale delivery mechanism and displayed little cytotoxicity toward the CCD 841 CoN reference cell line.

CONCLUSION:

These findings indicate that chitosan-based noisome encapsulation may enhance the effectiveness of CG-Nio-CGLD formulations in fighting cancer.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Colorectal Neoplasms / Chitosan / Liposomes / Antineoplastic Agents Limits: Humans Language: En Journal: BMC Pharmacol Toxicol Year: 2024 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Colorectal Neoplasms / Chitosan / Liposomes / Antineoplastic Agents Limits: Humans Language: En Journal: BMC Pharmacol Toxicol Year: 2024 Document type: Article Affiliation country: Country of publication: