Your browser doesn't support javascript.
loading
Enhancing chemotherapeutic efficacy: Niosome-encapsulated Dox-Cis with MUC-1 aptamer.
Barlas, Firat Baris; Olceroglu, Bilge; Ag Seleci, Didem; Gumus, Zinar Pinar; Siyah, Pinar; Dabbek, Meriam; Garnweitne, Georg; Stahl, Frank; Scheper, Thomas; Timur, Suna.
Affiliation
  • Barlas FB; Institute for Technical Chemistry, Leibniz University Hannover, Hannover, Germany.
  • Olceroglu B; Institue of Nanotechnology and Biotechnology, Istanbul University-Cerrahpasa, Istanbul, Turkey.
  • Ag Seleci D; Institue of Nanotechnology and Biotechnology, Istanbul University-Cerrahpasa, Istanbul, Turkey.
  • Gumus ZP; Institute for Particle Technology (iPAT), Technische Universität Braunschweig, Braunschweig, Germany.
  • Siyah P; Central Research Test and Analysis Laboratory Application and Research Center, Ege University, Izmir, Turkey.
  • Dabbek M; Department of Biochemistry, School of Pharmacy, Bahçesehir University, Istanbul, Turkey.
  • Garnweitne G; Institute for Technical Chemistry, Leibniz University Hannover, Hannover, Germany.
  • Stahl F; Institute for Particle Technology (iPAT), Technische Universität Braunschweig, Braunschweig, Germany.
  • Scheper T; Institute for Technical Chemistry, Leibniz University Hannover, Hannover, Germany.
  • Timur S; Institute for Technical Chemistry, Leibniz University Hannover, Hannover, Germany.
Cancer Med ; 13(15): e70079, 2024 Aug.
Article in En | MEDLINE | ID: mdl-39118454
ABSTRACT

BACKGROUND:

Cancer remains a formidable global health challenge, currently affecting nearly 20 million individuals worldwide. Due to the absence of universally effective treatments, ongoing research explores diverse strategies to combat this disease. Recent efforts have concentrated on developing combined drug regimens and targeted therapeutic approaches.

OBJECTIVE:

This study aimed to investigate the anticancer efficacy of a conjugated drug system, consisting of doxorubicin and cisplatin (Dox-Cis), encapsulated within niosomes and modified with MUC-1 aptamers to enhance biocompatibility and target specific cancer cells.

METHODS:

The chemical structure of the Dox-Cis conjugate was characterized using Fourier Transform Infrared Spectroscopy (FTIR) and Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry (LC-Q-TOF/MS). The zeta potential and morphological parameters of the niosomal vesicles were determined through Dynamic Light Scattering (DLS) and Transmission Electron Microscopy (TEM). In vitro assessments of cell viability and apoptosis were conducted on MUC-1 positive HeLa cells and MUC-1 negative U87 cells.

RESULTS:

The findings confirmed the successful conjugation of Dox and Cis within the niosomes. The Nio/Dox-Cis/MUC-1 formulation demonstrated enhanced efficacy compared to the individual drugs and their unencapsulated combination in both cell lines. Notably, the Nio/Dox-Cis/MUC-1 formulation exhibited greater effectiveness on HeLa cells (38.503 ± 1.407) than on U87 cells (46.653 ± 1.297).

CONCLUSION:

The study underscores the potential of the Dox-Cis conjugate as a promising strategy for cancer treatment, particularly through platforms that facilitate targeted drug delivery to cancer cells. This targeted approach could lead to more effective and personalized cancer therapies.
Subject(s)
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Doxorubicin / Cell Survival / Cisplatin / Mucin-1 / Aptamers, Nucleotide / Liposomes Limits: Humans Language: En Journal: Cancer Med Year: 2024 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Doxorubicin / Cell Survival / Cisplatin / Mucin-1 / Aptamers, Nucleotide / Liposomes Limits: Humans Language: En Journal: Cancer Med Year: 2024 Document type: Article Affiliation country: Country of publication: