Your browser doesn't support javascript.
loading
Multiplexed Nanoscopy via Buffer Exchange.
Chang, Ting-Jui Ben; Yang, T Tony.
Affiliation
  • Chang TB; Department of Electrical Engineering, National Taiwan University, Taipei 10617, Taiwan.
  • Yang TT; Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei 10617, Taiwan.
ACS Nano ; 18(34): 23445-23456, 2024 Aug 27.
Article in En | MEDLINE | ID: mdl-39143924
ABSTRACT
Understanding cellular functions, particularly in their intricate complexity, can greatly benefit from the spatial mapping of diverse molecules through multitarget single-molecule localization microscopy (SMLM). Existing methodologies, primarily restricting the encoding dimensions to color and lifetime or requiring cyclic staining, often involve broad chromatic detection, specialized optical configurations, or sophisticated labeling techniques. Here, we propose a simple approach called buffer-exchange stochastic optical reconstruction microscopy (beSTORM), which introduces an additional dimension to differentiate between single molecules irrespective of their spectral properties. This method leverages the distinguishable photoblinking responses to distinct buffer conditions, offering a straightforward yet effective means of fluorophore discrimination. Through buffer exchanges, beSTORM achieves multitarget SMLM imaging with minimal crosstalk. Direct integration with expansion microscopy (ExM) demonstrates its capability to resolve up to six proteins at the molecular level within a single emission color without chromatic aberration. Overall, beSTORM presents a highly compatible imaging platform, promising significant advancements in highly multiplexed nanoscopy for exploring multiple targets in biological systems with nanoscale precision.
Subject(s)
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Single Molecule Imaging Language: En Journal: ACS Nano Year: 2024 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Single Molecule Imaging Language: En Journal: ACS Nano Year: 2024 Document type: Article Affiliation country: Country of publication: