Effects of depression and insomnia on HPA axis and amino acid and monoamine neurotransmitters in hypothalamus of rats / 中国药理学通报
Chinese Pharmacological Bulletin
; (12): 815-822, 2021.
Article
in Zh
| WPRIM
| ID: wpr-1014441
Responsible library:
WPRO
ABSTRACT
Aim To explore the effects of chronic unpredictable mild stress combined with sleep deprivation on the hypothalamic-pituitary-adrenal axis (HPA) and the amino acid and monoamine transmitters in hypothalamus of rats. Methods Chronic unpredictable mild stress combined with modified multi-platform water environment sleep deprivation was used to replicate depression and insomnia rat models. ELISA was used to measure the content of HPA axis related molecules in serum and hypothalamus, the content of amino acid transmitter glutamate (Glu) and GABA in hypothalamus; HPLC-ECD was applied to measure the content of monoamine transmitter NE, 5-HT, DA in hypothalamus; WB and RT-qPCR were employed to measure the expression of GABA related molecules GAD67, GABA
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Chinese Pharmacological Bulletin
Year:
2021
Document type:
Article