The effects of gastrodin on TLR4 expression in microglia induced by ischemia and hypoxia / 神经解剖学杂志
Chinese Journal of Neuroanatomy
; (6): 50-56, 2024.
Article
in Zh
| WPRIM
| ID: wpr-1019636
Responsible library:
WPRO
ABSTRACT
Objective:
To determine the effect of gastrodin(GAS)on toll-like receptor 4(TLR4)expression in mi-croglia after hypoxic-ischemic brain damage.Methods:
Hypoxia-ischemic brain damage(HIBD)model was established in neonatal rat in vivo.Thirty 3 d SD rats of were randomly divided into there groupsSham group,HIBD model group,HIBD model+gastrodin intervention group(HIBD+G).Oxygen glucose deprivation(OGD)model was established in BV2 cells in vitro,Control group(Control),oxygen glucose deprivation group(OGD),OGD+gastrodin intervention group(OGD+G)were randomly set in vitro.Both Western Blot and immunofluorescence staining techniques were used to detect the expression of TLR4 in cells of each group in vitro and in the left corpus callosum region in vivo.Results:
The expression of TLR4 was significantly increased in OGD-induced microglia.After gastrodin intervention,TLR4 expression was decreased significantly(P<0.05).Conclusion:
GAS can inhibit the expression of TLR4 in activated microglia and thus play a neuroprotective role in HIBD.
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Neuroanatomy
Year:
2024
Document type:
Article