Correlation of MR diffusion imaging and triphenyl tetrazolium chloride staining in rats after focal cerebral ischemia and reperfusion / 中华神经医学杂志

ABSTRACT

Objective To investigate the changes of MR diffusion imaging (DWI) appearance in rats after focal cerebral ischemia reperfusion,and explore its relation with the changes of triphenyl tetrazolium chloride (TTC) staining.Methods Forty-four male Sprague Dawley rats were randomly divided into a sham-operated group (n=4) and two experimental groups (n=20,ischemia for 2 and 6 h,respectively); rats in the experimental groups were performed occlusion of the right middle cerebral artery (MCAO) with thread,and reperfusion for 0,0.5,2,6 and 24 h (n=4).DWI was performed at each time point; and then,the rats were sacrificed and TTC staining was performed.Results Before reperfusion,DWI demonstrated hyperintensity in the right hemisphere in the experimental groups,the ADC value was decreased,and white area was noted by TTC staining.The experimental group of ischemia for 6 h had larger area of DWI abnormal signal and decreased apparent diffusion cofficient (ADC) value as compared with those in the experimental group of ischemia for 2 h (P＜0.05).In experimental group of ischemia for 2 h,area of DWI abnormal signal shrank 24 h after reperfusion (P＜0.05),but that was slightly diminished in experimental group of ischemia for 6 h.No marked difference of abnormal area was noted between those shown by DWI and TTC staining (P＞0.05).Conclusion DWI can reflect the infarction area in rats after focal cerebral ischemia reperfusion.