IL-15 Induced an Increased SDF-1 Expression in the Synovial Fibroblasts of Patients with Rheumatoid Arthritis / 대한류마티스학회지
The Journal of the Korean Rheumatism Association
; : 238-245, 2010.
Article
in Ko
| WPRIM
| ID: wpr-137467
Responsible library:
WPRO
ABSTRACT
OBJECTIVE:
Interleukin-15 (IL-15) recruits and activates synovial T cells, and IL-15 plays an important role in amplifying and perpetuating inflammation in the pathogenesis of rheumatoid arthritis (RA). Stromal cell-derived factor-1 (SDF-1) is a potent chemoattractant for memory T cells in the inflamed RA synovium. This study investigated the effect of IL-15 on SDF-1 production in RA fibroblast-like synoviocytes (FLS).METHODS:
The expressions of IL-15 and SDF-1 were determined from the synovium of patients with RA and osteoarthritis (OA) by performing immunohistochemistry. The expressions of SDF-1 was measured from the RA FLS that were cultured with IL-15 and IL-17 by real-time RT-PCR and ELISA. The SDF-1 expression was also measured, via ELISA, from the RA FLS stimulated by IL-15 together with the inhibitors of such intracellular signal molecules as phosphatidylinositol 3-kinase (PI 3-kinase, LY294002), STAT3 (AG490), MAP Kinase (PD98059), NF-kappaB (parthenolide) and activator protein 1 (AP-1, curcumin).RESULTS:
IL-15 and SDF-1 were mainly expressed in the RA synovium compared to that of the OA synovium. IL-15 increased the SDF-1 expressions and it, and had an additive effect with IL-17 on the SDF-1 expressions in the cultured RA FLS. The IL-15 induced increase of the SDF-1 expression in the cultured RA FLS was blocked by the inhibitors of PI 3-kinase, NF-kappaB and AP-1.CONCLUSION:
The SDF-1 expression was increased in the RA synovium and it was up-regulated by IL-15 in the RA FLS through the PI 3-kinase, NF-kappaB, and AP-1 pathways. These results imply that the IL-15 induced increase of the SDF-1 expressions may be involved in the immunopathogenesis of RA.Key words
Full text:
1
Database:
WPRIM
Main subject:
Osteoarthritis
/
Phosphotransferases
/
Arthritis, Rheumatoid
/
Synovial Membrane
/
Enzyme-Linked Immunosorbent Assay
/
Immunohistochemistry
/
T-Lymphocytes
/
NF-kappa B
/
Transcription Factor AP-1
/
Interleukin-15
Limits:
Humans
Language:
Ko
Journal:
The Journal of the Korean Rheumatism Association
Year:
2010
Document type:
Article