Effect of Genistein on the Survival and Production of Nitric Oxide in Trabecular Meshwork Cells
Journal of the Korean Ophthalmological Society
; : 970-974, 2011.
Article
in Ko
| WPRIM
| ID: wpr-186834
Responsible library:
WPRO
ABSTRACT
PURPOSE:
To investigate the effects of genistein on the survival and production of nitric oxide in cultured human trabecular meshwork cells (HTMC).METHODS:
Primarily cultured HTMC were exposed to 0, 1, and 10 microm genistein using serum-deprived media. Production of nitric oxide and eNOS activity were assessed with the Griess assay and RT-PCR after exposure to genistein for 10 min and one day. Cellular survival was assessed via MTT assay after exposure to genistein for one day.RESULTS:
Genistein significantly increased the production of nitric oxide after exposure for 1 min at 10 microm and for 1 day at 1 microm under serum-deprived conditions. Genistein increased eNOS activity and cellular survival in HTMC.CONCLUSIONS:
Genistein increases cellular survival under serum-deprived conditions, accompanied with an increase in nitric oxide production after both short-term and long-term exposures.Key words
Full text:
1
Database:
WPRIM
Main subject:
Trabecular Meshwork
/
Genistein
/
Nitric Oxide
Limits:
Humans
Language:
Ko
Journal:
Journal of the Korean Ophthalmological Society
Year:
2011
Document type:
Article