Effect of Yangjing Zhongyu Decoction on mRNA and protein expression of PCNA, StAR, and FSHR in ovarian granulosa cells cultured by excess androgen / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
; (12): 312-316, 2014.
Article
in Zh
| WPRIM
| ID: wpr-312824
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of Yangjing Zhongyu Decoction (YZD) on mRNA and protein expression of PCNA, StAR, and FSHR in ovarian granulose cells (GCs) cultured by excess androgen.</p><p><b>METHODS</b>Ovarian GCs from porcine follicles were isolated and cultured in vitro. Follicular stimulating hormone (FSH) or YZD was added in the GCs treated by excess testosterone propionate. Totally 48 h later mRNA and protein expression of PCNA, StAR, and FSHR were detected by RT-PCR and Western blot.</p><p><b>RESULTS</b>Excess androgen inhibited mRNA and protein expression of PCNA, StAR, and FSHR of GCs. FSH and YZD could antagonize inhibition of excess androgens, and promote mRNA and protein expression of PCNA, StAR, and FSHR in GCs.</p><p><b>CONCLUSION</b>YZD could antagonize the inhibition of excess androgen on mRNA and protein expression of PCNA, StAR and FSHR in GCs. Thus, we inferred that YZD could improve the follicle dysplasia by promoting mRNA and protein expression of PCNA, StAR and FSHR in GCs.</p>
Full text:
1
Database:
WPRIM
Main subject:
Membrane Transport Proteins
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Pharmacology
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Swine
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Receptors, FSH
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RNA, Messenger
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Drugs, Chinese Herbal
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Cells, Cultured
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Proliferating Cell Nuclear Antigen
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Cell Biology
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Follicle Stimulating Hormone
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2014
Document type:
Article