Mechanisms of G2/M cycle arrest induced by topo IIalpha and II beta inhibitors in H460 cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 2187-2190, 2008.
Article
in Zh
| WPRIM
| ID: wpr-321733
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To compare the mechanisms of G(2)/M cycle arrest induced by topo IIalpha and IIbeta inhibitors in H460 cells.</p><p><b>METHODS</b>The inhibitory effects of XK469, adriamycin and etoposide on H460 cell growth were analyzed by MTT assay. The changes in cell cycle and expressions of cdc2, phos-cdc2 and 14-3-3sigma proteins induced by these 3 topo II inhibitors were detected by flow cytometry and Western blotting, respectively.</p><p><b>RESULTS</b>Both of the two types of topo II inhibitor resulted in dose-dependent G(2)/M phase arrest and growth inhibition of H460 cells, but XK469 failed to induce 14-3-3sigma protein expression as adriamycin and etoposide did.</p><p><b>CONCLUSION</b>Topo IIalpha and topo IIbeta inhibitors induce growth inhibition of H460 cells possibly through two different mechanisms, namely the 14-3-3sigma-dependent pathway and the 14-3-3sigma-independent pathway, but further functional inhibition test of 14-3-3sigma is needed to confirm this hypothesis.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pathology
/
Pharmacology
/
Physiology
/
Quinoxalines
/
Cell Cycle
/
Cell Division
/
G2 Phase
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Carcinoma, Non-Small-Cell Lung
/
DNA Topoisomerases, Type II
/
Cell Line, Tumor
Limits:
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2008
Document type:
Article