Kinetics of Cu crossing human erythrocyte membrane / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1707-1710, 2014.
Article
in Zh
| WPRIM
| ID: wpr-340432
Responsible library:
WPRO
ABSTRACT
This study was aimed to investigate various factors influencing the proceduction of Cu(II) crossing human erythrocyte membrane, including concentration of Cu²⁺, pH value of the medium, temperature and time of incubation, and to derive kinetic equation of Cu(II) crossing human erythrocyte membrane. Suspension red blood cells were incubated by Cu²⁺, then content of Cu²⁺ crossed human erythrocyte membrane was determined by atomic absorption spectrometry under various conditions after digestion. The results showed that content of Cu²⁺ crossed human erythrocyte membrane increased with the increase of extracellular Cu²⁺ and enhancement of incubation temperature, and the content of Cu²⁺ crossed human erythrocyte membrane showed a increasing tendency when pH reached to 6.2-7.4, and to maximum at pH 7.4, then gradually decreased at range of pH 7.4-9.2. It is concluded that the Cu²⁺ crossing human erythrocyte has been confirmed to be the first order kinetics characteristics within 120 min, and the linear equation is 10³ × Y = 0.0497t +6.5992.
Full text:
1
Database:
WPRIM
Main subject:
Pharmacology
/
Temperature
/
Kinetics
/
Copper
/
Erythrocyte Membrane
/
Hydrogen-Ion Concentration
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2014
Document type:
Article