Effects of Ac-SDKP on angiotensin II-induced collagen synthesis in vascular adventitial fibroblasts / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 179-192, 2013.
Article
in Zh
| WPRIM
| ID: wpr-358645
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of an anti-fibrotic tetra peptide Ac-SDKP on vascular fibrosis by regulating extracellular regulated protein kinase (ERK1/2) activity through Ang II.</p><p><b>METHODS</b>Rat vascular adventitial fibroblasts were cultured in vitro. They were randomly divided into control group, Ang II (10(-6) mmol/L) group, Ang II and Ac-SDKP joint action group, PD98059 group. Type I, III collagen contents in adventitia fibroblasts were measured by RT-PCR and the expressions of matrix metalloproteinases (MMP-2) and transforming growth factor-beta1 (TGF-beta1) were determined by Western blot.</p><p><b>RESULTS</b>Ac-SDKP could reduced Ang II-induced expression of type I, III collagen secretion and TGF-beta1 at mRNA,and increase MMP-2 expression, PD98059 could inhibit the above effect.</p><p><b>CONCLUSION</b>The results suggested that Ac-SDKP could inhibit the formation and development of vascular fibrosis through blocking ERK1/2 pathway mediated by Ang II. Ac-SDKP therefore served as an antifibrotic factor in vascular fibrosis.</p>
Full text:
1
Database:
WPRIM
Main subject:
Oligopeptides
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Pharmacology
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Flavonoids
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Angiotensin II
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Cells, Cultured
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Rats, Sprague-Dawley
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Matrix Metalloproteinase 2
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MAP Kinase Signaling System
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Cell Biology
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Collagen Type I
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Applied Physiology
Year:
2013
Document type:
Article