Establishment of nasopharyngeal carcinoma (NPC) cell lines stable expressing NPC-derived LMP1 / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)1986.
Article
in Zh
| WPRIM
| ID: wpr-517505
Responsible library:
WPRO
ABSTRACT
AIM: To establish nasopharyngeal carcinoma(NPC) cell lines stable expressing NPC-derived latent membrane protein 1(LMP1) gene. METHODS: General expression vector and epithelium-specific expression vector of NPC-derived LMP1 gene were constructed by using recombinant techniques, then transfected these vectors into a poor differentiated NPC cell line named CNE-2 ,integration and expression of N-LMP1 in CNE-2 cells were detected by PCR,RT-PCR and Western blot. RESULTS: (1) General expression vector and epithelium-specific expression vector of NPC-derived LMP1 gene were constructed successfully.(2) It showed that N-LMP1 gene expressed in CNE-2 cells correctly. CONCLUSION: The first NPC cell lines which stable express NPC-LMP1 were established. The cell lines obtained will provide important basis for exploring the role of NPC-LMP1 in nasopharynx carcinogenesis.
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Pathophysiology
Year:
1986
Document type:
Article