HPLC-ELSD in determination of AR-Ⅰ content in total furostanol saponins of Asparagus cochinchinensis / 第二军医大学学报

ABSTRACT

Objective:To determine AR-Ⅰ content in total furostanol saponins of Asparagus cochinchinensis.Methods: High performance liquid chromatography(HPLC) was used and the condition was: Diamonsil C_(18) column(5 mm?250 mm,5 ?m);mobile phase:(CH_(3)CN-H_(2)O(36∶64);) flow rate: 0.8 ml/min;injector volume:20 ?l;colum temperature: room temperature.Evaporative light scattering detector(ELSD) condition was: drift tube temperature: 40℃;and nebulizer gas pressure(N_(2)): 2.0?10~(5) Pa.Results: The linearity was good when AR-I was within the range of 0.094-0.940 0 g?L~(-1)(r=0.999 2).The test had higher sensitivity and good stability.The average recovery was 101.3%(n=6)and RSD was 2.36%.Conclusion: HPLC-ELSD is practical and reliable for the determination of the furostanol saponins of Asparagus cochinchinensis.